Journal: Metabolic Engineering Communications
Article Title: Multi-layered metabolic remodeling of Pseudomonas putida for efficient conversion of lignocellulosic sugars to the precursors of advanced aviation fuel
doi: 10.1016/j.mec.2026.e00274
Figure Lengend Snippet: Fed-batch cultivation of PIPAxyl-E3-K3-O15 for isoprenyl acetate production. (a) Residual sugar concentrations and feeding rate over time. The culture was initiated with 13.3 g/L glucose and 6.7 g/L xylose and continuously fed with a solution containing 266.7 g/L glucose and 133.3 g/L xylose. (b) Time-course profile of isoprenyl acetate production. Titers represent the sum of isoprenyl acetate quantified from the aqueous phase, the Durasyn overlay in the bioreactor, and the Durasyn off-gas trap. Cultivations were performed with 1 L medium and 200 mL overlay with sampling approximately every 12 h. Arabinose, salicylic acid, and m -toluic acid were added at OD 600 0.6–0.8 to final concentrations of 2 g/L, 62.5 μM, and 250 μM, respectively.
Article Snippet: Fed-batch cultivation was conducted in a 2 L DASGIP parallel bioreactor system (Eppendorf) with a working volume of 1.2 L. Seed cultures were prepared through a three-step adaptation.
Techniques: Sampling